TY - JOUR AU - Kitagawa, Sayuri AU - Jing Lin Wong, Jack AU - Matsuoka, Tatsuo AU - Nakao, Yoshihiro AU - Nakai, Masaaki PY - 2023/12/28 Y2 - 2026/03/01 TI - In Vitro Anti-oxidation, Anti-inflammatory and Anti-aging activities of Oak Extract JF - Asian Medical Journal and Alternative Medicine JA - AMJAM VL - 23 IS - 3 SE - Original Articles DO - UR - https://asianmedjam.com/index.php/amjam/article/view/1432 SP - 44-52 AB - <p><strong>Introduction</strong><strong>:</strong> Intrinsic and extrinsic factors can influence skin aging. Intrinsic skin aging can occur due to the formation and accumulation of reactive oxygen species, furthermore UV radiation contributes up to 80% of extrinsic aging. Anti-oxidation bioactive components from natural sources have been recommended for skin aging prevention.<br /><br /><strong>Objectives</strong><strong>:</strong> we explored the anti-oxidation, anti-inflammatory and anti-aging activities of oak extract (freeze-dried) product.<br /><br /><strong>Methods</strong><strong>:</strong> Anti-oxidation activity was determined using superoxide dismutase (SOD) activity assay and radical scavenging activity assay (DPPH). Anti-inflammatory activity was determined by hyaluronidase activity inhibitory assay and hexosaminidase release inhibitory assay. Anti-aging activity was determined using inhibitory assay of human neutrophil elastase activity, inhibitory assay of matrix metalloproteinase-1 (MMP-1) and promoting assay of hyaluronic acid production.<br /><br /><strong>Results</strong><strong>: </strong>Anti-oxidation activity was observed for oak extract in both SOD activity assay and radical scavenging activity assay with IC<sub>50 </sub>of 2.27µg/mL and 3.25 µg/mL respectively. Anti-inflammatory activity was detected in hyaluronidase activity inhibitory assay with an IC<sub>50 </sub>of &gt;400µg/ml and hexosaminidase release inhibitory effect was observed with IC<sub>50</sub> of 223.4 µg/mL. The oak extract also exhibited anti-aging activity with human neutrophil elastase activity inhibitory at an IC<sub>50</sub> of 20.87µg/mL and MMP-1 activity inhibitory effect at an IC<sub>50</sub> of 125.7µg/mL. Oak extract did not promote any hyaluronic acid production in epidermal keratinocytes.<br /><br /><strong>Conclusions</strong><strong>:</strong> Oak extract exhibits a strong antioxidant effect comparable to control Baicalin and ascorbic acid. Together with the inhibitory effect on human neutrophil elastase and MMP-1, these results suggest that oak extract is a promising agent as an anti-aging material.</p> ER -