TY  - JOUR
AU  - Jantalika, Tanachapa
AU  - Tantrawatpan, Chairat
AU  - Manochantr, Sirikul
AU  - Tantikanlayaporn, Duangrat
AU  - Kheolamai, Pakpoom
PY  - 2022/08/01
Y2  - 2026/03/01
TI  - Human Placenta-derived Mesenchymal Stem Cells  Inhibit Proliferation and Promote Apoptosis  in Cholangiocarcinoma Cell Lines via Suppressing  JAK2/STAT3 Signaling
JF  - Asian Medical Journal and Alternative Medicine
JA  - AMJAM
VL  - 22
IS  - -
SE  - Abstracts
DO  - 
UR  - https://asianmedjam.com/index.php/amjam/article/view/1332
SP  - S111
AB  - &lt;p&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Cholangiocarcinoma (CCA) is an aggressive malignancy originating from the bile duct. Incidence and mortality increase worldwide because of late diagnosis and a lack of therapeutic options. Mesenchymal stem cells (MSC) can secrete several cytokines to promote or inhibit the progression of many cancer cell types. However, the effect of human placenta-derived MSCs (PL-MSC) on CCA progression has not been clarified.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; The present study aims to investigate the effects and molecular mechanisms of conditioned media derived from PL-MSC (PL-CM) on three CCA cell lines (KKU100, KKU213A, and KKU213B).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods:&lt;/strong&gt; CCA cells were cultured with PL-CM to examine cell proliferation and apoptosis by MTT assay and annexin V/PI analysis, respectively. JC-1 staining, caspase 3 activity, and western blot were used to investigate the mechanism of PL-CM induced apoptosis of CCA cell lines through mitochondrial apoptotic pathway and JAK2/STAT3 signaling.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; PL-CM could suppress proliferation and promote apoptosis of all CCA cell lines by inducing mitochondrial apoptotic pathways, most likely through suppression of the JAK2/ STAT3 signaling pathway.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; This study suggests PL-CM has a potent anti-cancer effect on CCA cells. Therefore, PL-MSC may provide a basis for further clinical trials to study as well as a possible alternative treatment for CCA patients.&lt;/p&gt;
ER  -