TY  - JOUR
AU  - Chadee, Nuengruethai 
AU  - Suwanprateep, Jintamai 
AU  - Tantrawatpan, Chairat 
AU  - Kheolamai, Pakpoom 
AU  - Tantikanlayaporn, Duangrat 
AU  - Manochantr, Sirikul 
PY  - 2022/08/01
Y2  - 2026/03/01
TI  - The Expression of Alkaline Phosphatase  in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells
JF  - Asian Medical Journal and Alternative Medicine
JA  - AMJAM
VL  - 22
IS  - -
SE  - Abstracts
DO  - 
UR  - https://asianmedjam.com/index.php/amjam/article/view/1331
SP  - S110
AB  - <p><strong>Introduction:</strong> Mesenchymal stem cells (MSCs) have gained more interest in regenerative medicine. The umbilical cord, the alternative source of MSCs, has thus been increasingly recognized as a good MSC source for bone tissue regeneration and bone defect repair. Metformin, an anti-hyperglycemic drug, has been reported that promotes the osteogenic differentiation of BM-MSCs. <br><strong>Objectives:</strong> This study aims to investigate the viability and the expression of alkaline phosphatasein metformin-treated umbilical cord-derived MSCs (UC-MSCs) during osteogenicdifferentiation.<br><strong>Methods:</strong> The UC-MSCs were obtained from pregnant women after normal delivery. After charac-terization, UC-MSCs were cultured with different metformin concentrations (0 - 160 μM). The cell viability and alkaline phosphatase (ALP) expression were observed by MTT and alkaline phosphatase activity assay.<br><strong>Results:</strong> The result showed that metformin at a concentration of 0 - 160 μM did not decrease the viability of UC-MSCs. Interestingly, the alkaline phosphatase activity of metformin-treated UC-MSCs was significantly increased, compared with untreated UC-MSCs in a dose-dependent manner. <br><strong>Conclusions:</strong> The data suggested that metformin could enhance the expression of alkaline phosphatase and the osteogenic differentiation ability of MSCs derived from the umbilical cord.</p>
ER  -