TY  - JOUR
AU  - Suwanmanee, Gunticha
AU  - Tantrawatpan, Chairat
AU  - Kheolamai, Pakpoom
AU  - Tantikanlayaporn, Duangrat
AU  - Manochantr, Sirikul
PY  - 2022/08/01
Y2  - 2026/03/01
TI  - Fucoxanthin Attenuates Hydrogen Peroxide-induced  Oxidative Stress in Placenta-derived Mesenchymal  Stem Cells through Antioxidant Activity
JF  - Asian Medical Journal and Alternative Medicine
JA  - AMJAM
VL  - 22
IS  - -
SE  - Abstracts
DO  - 
UR  - https://asianmedjam.com/index.php/amjam/article/view/1330
SP  - S109
AB  - &lt;p&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Osteoporosis is a bone disease that results in a loss of bone mass. Oxidative stress promotes osteoblast apoptosis which leads to decrease bone formation. Fucoxanthin, a carotenoid extracted from brown seaweeds, has antioxidant activity through an increased antioxidant enzyme expression.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; This study attempts to explore the cytoprotective effect and antioxidant activity of fucoxanthin to promote the cell viability of placenta-derived mesenchymal stem cells (PL-MSCs) under oxidative stress conditions.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods:&lt;/strong&gt; PL-MSCs were obtained from a pregnant woman after normal delivery. Following the characterization, PL-MSCs were treated with fucoxanthin (1-5 µM) and 750 µM H&lt;sub&gt;2&lt;/sub&gt; O&lt;sub&gt;2&lt;/sub&gt; for 24 h or cultured in both fucoxanthin (1-5 µM) and H&lt;sub&gt;2&lt;/sub&gt; O&lt;sub&gt;2&lt;/sub&gt; for 24 h. The cell viability, intracellular reactive oxygen species(ROS) production,superoxide dismutase (SOD) activity and glutathione (GSH) were determined following treatment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; The resultsshowed that treatment and pretreatment with fucoxanthin increased cell viability, SOD activity, GSH levels of PL-MSCs when compare to control which cultured in 750 µM H&lt;sub&gt;2&lt;/sub&gt; O&lt;sub&gt;2&lt;/sub&gt; without fucoxanthin for 24 h. While, treatment and pretreatment with fucoxanthin decreased intracellular ROS production of PL-MSCs when compare to control which cultured in 750 µM H&lt;sub&gt;2&lt;/sub&gt; O&lt;sub&gt;2&lt;/sub&gt; without fucoxanthin for 24 hours.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; This study demonstrated that the treatment and pretreatment with fucoxanthin increased the viability and increased antioxidant activity of PL-MSCs when compared with the control which cultured in 750 µM H&lt;sub&gt;2&lt;/sub&gt; O&lt;sub&gt;2&lt;/sub&gt; without fucoxanthin. In addition, the treatment with fucoxanthin decreased intracellular ROS production compared with the control. This study established that fucoxanthin can protect PL-MSCs against oxidative stress.&lt;/p&gt;
ER  -