TY  - JOUR
AU  - Sukkasem, Kanmanee
AU  - Itharat, Arunporn
AU  - Thisayakorn, Krittiya
AU  - Panthong, Sumalee
AU  - Makchuchit, Sunita
AU  - Inprasit, Janjira
AU  - Prommee, Nuntika
AU  - Khoenok, Wicheian
AU  - Sriyam, Kanjana
AU  - Pahusee, Darunee
PY  - 2022/08/01
Y2  - 2026/03/01
TI  - Anti-inflammatory Activity of Kheaw-Hom Remedy  in Lipopolysaccharide-stimulated Macrophage Cells  and Carrageenan-induced Paw Edema in Rats
JF  - Asian Medical Journal and Alternative Medicine
JA  - AMJAM
VL  - 22
IS  - -
SE  - Abstracts
DO  - 
UR  - https://asianmedjam.com/index.php/amjam/article/view/1327
SP  - S108
AB  - &lt;p&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; Kheaw-Hom (KH) remedy has been widely used to treat inflammation and fever in children with measles and chickenpox. However, its anti-inflammatory activity, both &lt;em&gt;in vitro&lt;/em&gt; and&lt;em&gt; in vivo&lt;/em&gt;, has not been scientifically tested.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; To evaluate the&lt;em&gt; in vitro&lt;/em&gt; and &lt;em&gt;in vivo&lt;/em&gt; anti-inflammatory activities of KH remedy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods:&lt;/strong&gt; The &lt;em&gt;in vitro&lt;/em&gt; anti-inflammatory activities of the aqueous extract (KHA) and ethanolic extract (KHE) were investigated using lipopolysaccharide (LPS)-stimulated nitric oxide (NO), prostaglandin E&lt;sub&gt;2&lt;/sub&gt; (PGE&lt;sub&gt;2&lt;/sub&gt; ), and tumor necrosis factor-alpha (TNF-α) in macrophage RAW 264.7 cells. The &lt;em&gt;in vivo&lt;/em&gt; anti-inflammatory activity of KHE and KH powder (KHP) was determined using carrageenan-induced paw edema in rats and the PGE&lt;sub&gt;2&lt;/sub&gt; production in tissue samples was examined using ELISA kit.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; KHE exhibited anti-inflammatory activities through the suppression of PGE&lt;sub&gt;2&lt;/sub&gt; , NO, and TNF-α production with IC&lt;sub&gt;50&lt;/sub&gt; values of 19.69 ± 2.09, 39.93 ± 3.28, and 85.07 ± 2.61 μg/ mL, respectively, while KHA did not. Interestingly, KHP orally administered at the dose of 100 mg/kg significantly reduced rat paw edema at 1, 2, and 3 hours (37.25%, 25.00%, and 27.09%, respectively) while KHE at the dose of 100 mg/kg noticeably reduced at 2 and 3 hours (21.67% and 19.49%) after carrageenan injection. KHP at the dose of 100 mg/kg showed maximum inhibition of PGE&lt;sub&gt;2&lt;/sub&gt; production with the PGE&lt;sub&gt;2&lt;/sub&gt; levels of 15.46 ± 1.33 ng/mL.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; The findings from &lt;em&gt;in vivo&lt;/em&gt; study strongly parallel the results of the&lt;em&gt; in vitro&lt;/em&gt; study and support the traditional use of KH as a potent anti-inflammatory and antipyretic remedy.&lt;/p&gt;
ER  -