TY  - JOUR
AU  - Chularojmontri, Linda 
AU  - Nanna, Urarat 
PY  - 2022/08/01
Y2  - 2026/03/01
TI  - Effect of Carallia brachiata Ethanolic Extract  on Adipogenesis in 3T3-L1 Adipocytes
JF  - Asian Medical Journal and Alternative Medicine
JA  - AMJAM
VL  - 22
IS  - -
SE  - Abstracts
DO  - 
UR  - https://asianmedjam.com/index.php/amjam/article/view/1305
SP  - S126
AB  - &lt;p&gt;&lt;strong&gt;Introduction:&lt;/strong&gt; An excessstorage of body fat causes obesity. Since obesity increasesrisk of chronic diseases, thusit isimportant to inhibit excessive storage of fat. &lt;em&gt;Carallia brachiata&lt;/em&gt; is a plant that found in Thailand. There is a report that the leaf of &lt;em&gt;Carallia brachiata&lt;/em&gt; has an antidiabetic effect in rat model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objectives:&lt;/strong&gt; The aim of this study was to demonstrate the effect of &lt;em&gt;Carallia brachiata&lt;/em&gt; leaf and stem ethanolic extracts (CL and CS) on adipogenesis in 3T3-L1 adipocytes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods:&lt;/strong&gt; 3T3-L1 adipocytes were used for measuring cytotoxicity of CL and CS (3, 10, 30, and 100 μg/mL). To determine adipogenesis process, CL and CS extracts were added to the cell culture medium at concentrations of 0, 3, 10, 30 and 100 μg/mL. After 8 days of treatments, adipocyte cells were stained with Oil Red O solution and measured the expression of adipogenic genes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results:&lt;/strong&gt; CL and CS did not inhibit cell proliferation and showed no cytotoxicity in 3T3-L1 cells. Therefore, concentration range of 3 - 100 μg/mL CL and CS was used for subsequent experiments. CS inhibited lipid accumulation in 3T3-L1 adipocytes and suppressed gene&lt;br /&gt;expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein 1c (SREBP1c), adipocyte-specific genes fatty acid synthase (FAS), lipoprotein lipase (LPL) and adipocyte fatty acid-binding protein 2 (aP2). Whereas, CL dis not inhibit lipid storage and only had an inhibitory activity on SREBP1c and aP2 genes.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; Our findings suggest that &lt;em&gt;Carallia brachiata&lt;/em&gt; has an inhibitory effect on adipogenesis could be partially caused by suppressing C/EBPα, PPARγ and SREBP1c genes in 3T3-L1 adipocytes&lt;/p&gt;
ER  -