@article{Saesiw_Ruangnoo_Itharat_Kuropakornpong_Poomirat_Makchuchit_Sriumpai_Tontan_2024, title={Quality Control and Evaluation of Anti-inflammatory and Antioxidant Activities of Ethanolic Extracts from Ha-Rak remedy, Piper betle Linn., Garcinia mangostana Linn., and Their Combined Remedies}, volume={24}, url={https://asianmedjam.com/index.php/amjam/article/view/1471}, abstractNote={<p><strong>Introduction:</strong> A Thai herbal remedy, Ha-Rak (HR), Piper betle Linn. (PB) leaves, and Garcinia mangostana Linn. (GM) pericarps tend to relieve inflammatory related atopic dermatitis (AD). Additionally, antioxidants have important role in the AD prevention.</p>
<p><strong>Objectives:</strong> To develop combined formulas of HR, PB and GM extracts and investigate them on anti-inflammatory and antioxidant activities, the total flavonoid content (TFC) and the total phenolic content (TPC).</p>
<p><strong>Methods:</strong> The quality of each dried plant material was evaluated according to Thai Herbal Pharmacopoeia (THP) criteria. Each was extracted by maceration with 95% ethanol, and then combined with a remedy. The inflammatory properties of each extract were assessed using the production inhibition of TNF- α from RAW 264.7 cells. The antioxidant properties were assessed using DPPH and ABTS radical scavenging. The TFC and TPC were also analyzed.</p>
<p><strong>Results:</strong> The quality of all plant materials passed Thai herbal pharmacopeia standards. HR showed the highest anti inflammatory activity. The combination HMB-321, which is composed of HR:GM:PB in a ratio of 3:2:1, showed the highest anti-inflammatory activity among other combined formulas. Moreover, HMB-321 showed moderate antioxidant activities and capacities. PB showed the highest antioxidant activity but less anti-inflammatory activity. HMB-123, whose proportion of HR:GM:PB was 1:2:3, showed the highest antioxidant activity among the combination of extracts but showed less anti-inflammatory activity.</p>
<p><strong>Conclusions:</strong> The combination HMB-321 could be a candidate remedy for the prevention and treatment of AD. Further study should be directed to in vivo testing and clinical trials for anti-inflammatory and antioxidant activities.</p>}, number={2}, journal={Asian Medical Journal and Alternative Medicine}, author={Saesiw, Ubonwan and Ruangnoo, Srisopa and Itharat, Arunporn and Kuropakornpong, Pranporn and Poomirat, Saovapak and Makchuchit, Sunita and Sriumpai, Pattama and Tontan, Jagavet}, year={2024}, month={Aug.}, pages={33–44} }