@article{Pipatrattanaseree_Thaptimthong_Boonrasri_Rattanajarasroj_Ontong_Chaisomboonpan_2023, title={Potential in vitro Anti-inflammatory and Anti-oxidant Activities of Various Extracts of Etlingera elatior Inflorescences.}, volume={23}, url={https://asianmedjam.com/index.php/amjam/article/view/1443}, abstractNote={<p><strong>Introduction:</strong> <em>Etlingera elatior </em>(Jack) Smith, an edible plant in the Zingiberaceae family, used as food and folk medicine for earaches and wound cleansing. Although some parts of the plant have been studied for their anti-inflammatory properties, however, there is a lack of research on the inhibition of nitric oxide (NO), prostaglandin E<sub>2</sub> (PGE<sub>2</sub>), and tumor necrosis factor α (TNF-α) production of the inflorescences. The findings demonstrated its anti-inflammatory effects and suggested an effective extraction method for further investigation, quality control, and product development.</p>
<p><strong>Objectives: </strong>This study aims to explore the anti-inflammatory and antioxidant properties of <em>E. elatior</em> inflorescence extracts.</p>
<p><strong>Methods: </strong>The bracts of <em>E. elatior </em>inflorescences were dried and separately extracted by 95%, 70%, and 50%ethanol maceration and decoction. The anti-inflammatory activity was investigated by inhibiting the production of nitric oxide (NO), prostaglandin E2 (PGE<sub>2</sub>), and tumor necrosis factor (TNF-α) in lipopolysaccharide-induced RAW264.7 cells. Additionally, the anti-oxidant activity was assessed by DPPH scavenging assay.</p>
<p><strong>Results: </strong>The 70%ethanolic extract of <em>E. elatior </em>exhibited both inhibitory effect on NO production (IC<sub>50</sub> = 16.36 µg/mL) and DPPH scavenging activity (EC<sub>50</sub> = 23.78 µg/mL), whereas the 95% ethanolic showed comparable inhibitory activity on NO (IC<sub>50</sub> = 16.78 µg/mL) but not in antioxidant activity. EE95 also showed moderate inhibition of PGE<sub>2</sub> production (IC<sub>50</sub> = 45.26 µg/mL).</p>
<p><strong>Conclusion: </strong>The ethanolic extracts of <em>E. elatior</em> inflorescences, obtained through maceration with 70% ethanol and 95% ethanol exhibited remarkable anti-inflammatory and anti-oxidant properties. Consequently, it is imperative to further explore the 95% and 70% ethanolic extracts through in-depth research, including <em>in vivo</em> studies, phytochemical analysis, and anti-inflammatory product development.</p>}, number={3}, journal={Asian Medical Journal and Alternative Medicine}, author={Pipatrattanaseree, Weerachai and Thaptimthong, Thitiporn and Boonrasri, Narumon and Rattanajarasroj, Sadudee and Ontong, Sakwichai and Chaisomboonpan, Siriwan}, year={2023}, month={Dec.}, pages={64–71} }