@article{Kitagawa_Jing Lin Wong_Matsuoka_Nakao_Nakai_2023, title={In Vitro Anti-oxidation, Anti-inflammatory and Anti-aging activities of Oak Extract}, volume={23}, url={https://asianmedjam.com/index.php/amjam/article/view/1432}, abstractNote={<p><strong>Introduction</strong><strong>:</strong> Intrinsic and extrinsic factors can influence skin aging. Intrinsic skin aging can occur due to the formation and accumulation of reactive oxygen species, furthermore UV radiation contributes up to 80% of extrinsic aging. Anti-oxidation bioactive components from natural sources have been recommended for skin aging prevention.<br /><br /><strong>Objectives</strong><strong>:</strong> we explored the anti-oxidation, anti-inflammatory and anti-aging activities of oak extract (freeze-dried) product.<br /><br /><strong>Methods</strong><strong>:</strong> Anti-oxidation activity was determined using superoxide dismutase (SOD) activity assay and radical scavenging activity assay (DPPH). Anti-inflammatory activity was determined by hyaluronidase activity inhibitory assay and hexosaminidase release inhibitory assay. Anti-aging activity was determined using inhibitory assay of human neutrophil elastase activity, inhibitory assay of matrix metalloproteinase-1 (MMP-1) and promoting assay of hyaluronic acid production.<br /><br /><strong>Results</strong><strong>: </strong>Anti-oxidation activity was observed for oak extract in both SOD activity assay and radical scavenging activity assay with IC<sub>50 </sub>of 2.27µg/mL and 3.25 µg/mL respectively. Anti-inflammatory activity was detected in hyaluronidase activity inhibitory assay with an IC<sub>50 </sub>of >400µg/ml and hexosaminidase release inhibitory effect was observed with IC<sub>50</sub> of 223.4 µg/mL. The oak extract also exhibited anti-aging activity with human neutrophil elastase activity inhibitory at an IC<sub>50</sub> of 20.87µg/mL and MMP-1 activity inhibitory effect at an IC<sub>50</sub> of 125.7µg/mL. Oak extract did not promote any hyaluronic acid production in epidermal keratinocytes.<br /><br /><strong>Conclusions</strong><strong>:</strong> Oak extract exhibits a strong antioxidant effect comparable to control Baicalin and ascorbic acid. Together with the inhibitory effect on human neutrophil elastase and MMP-1, these results suggest that oak extract is a promising agent as an anti-aging material.</p>}, number={3}, journal={Asian Medical Journal and Alternative Medicine}, author={Kitagawa, Sayuri and Jing Lin Wong, Jack and Matsuoka, Tatsuo and Nakao, Yoshihiro and Nakai, Masaaki}, year={2023}, month={Dec.}, pages={44–52} }