@article{Maki_Thongdeeying_Itharat_Pipatrattanaseree_Davies_2023, title={Cytotoxic Activity Against Liver Cancer and Cholangiocarcinoma Cells of Artemisia vulgaris L. Extract and Cirsimaritin, Its Isolated Compound}, volume={23}, url={https://asianmedjam.com/index.php/amjam/article/view/1364}, abstractNote={<p><strong>Introduction:</strong> Liver cancer is the most common cancer in Thailand. <em>Artemisia vulgaris</em> L. is one of Thai herbs used in many Thai traditional cancer recipes. The objective was to investigate cytotoxic activity against hepatocellular carcinoma (HepG2), cholangiocarcinoma (KKU-M156) cells of <em>Artemisia vulgaris</em> L. Extract, and its compound.</p>
<p><strong>Methods:</strong> The SRB assay was used to determine the cytotoxic activity. Bioassay guided isolation was used for isolating cytotoxic compound. </p>
<p><strong>Results:</strong> The ethanolic extract of<em> A</em>. <em>vulgaris</em> L. showed good cytotoxic activity against HepG2 and KKU-M156 (IC<sub>50</sub> =13.36 ± 0.45 and 17.07 ± 0.95 µg/mL, respectively), while it also showed cytotoxicity against normal cell lines (HaCaT, IC<sub>50</sub> = 27.30 ± 3.71 µg/mL). Vacuum liquid chromatography (VLC) in (Chloroform: Methanol) fraction showed the best cytotoxic. Cirsimaritin, the isolated compound from the active fraction showed better cytotoxic activity against HepG2 than KKU-M156 (IC<sub>50</sub> =1.82 ± 0.63 and 21.01 ± 0.84 µg/mL, respectively), while it had no cytotoxicity against normal cell lines (HaCaT) (IC50 >100 µg/mL). This compound showed selective cytotoxicity against HepG2 with selective index (SI) value of 54.94.</p>
<p><strong>Conclusions:</strong> Cirsimaritin showed selective cytotoxic activity against liver cancer cells with the IC<sub>50</sub> value less than 4 µg/mL and the SI value more than 50. In regards to NCI criteria which consider that the value of IC<sub>50</sub> of a good cytotoxic compound must be less than 4 µg/mL, therefore, it has the potential to be developed as an anti-cancer drug for liver cancer cells. It also demostrated selective cytotoxic activity against liver cancer cells better than cholangiocarcinoma.</p>}, number={1}, journal={Asian Medical Journal and Alternative Medicine}, author={Maki, Ponlawat and Thongdeeying, Pakakrong and Itharat, Arunporn and Pipatrattanaseree, Weerachai and Davies, Neal M.}, year={2023}, month={Apr.}, pages={17–24} }