@article{Chadee_Suwanprateep_Tantrawatpan_Kheolamai_Tantikanlayaporn_Manochantr_2022, title={The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells}, volume={22}, url={https://asianmedjam.com/index.php/amjam/article/view/1331}, abstractNote={<p><strong>Introduction:</strong> Mesenchymal stem cells (MSCs) have gained more interest in regenerative medicine. The umbilical cord, the alternative source of MSCs, has thus been increasingly recognized as a good MSC source for bone tissue regeneration and bone defect repair. Metformin, an anti-hyperglycemic drug, has been reported that promotes the osteogenic differentiation of BM-MSCs. <br><strong>Objectives:</strong> This study aims to investigate the viability and the expression of alkaline phosphatasein metformin-treated umbilical cord-derived MSCs (UC-MSCs) during osteogenicdifferentiation.<br><strong>Methods:</strong> The UC-MSCs were obtained from pregnant women after normal delivery. After charac-terization, UC-MSCs were cultured with different metformin concentrations (0 - 160 μM). The cell viability and alkaline phosphatase (ALP) expression were observed by MTT and alkaline phosphatase activity assay.<br><strong>Results:</strong> The result showed that metformin at a concentration of 0 - 160 μM did not decrease the viability of UC-MSCs. Interestingly, the alkaline phosphatase activity of metformin-treated UC-MSCs was significantly increased, compared with untreated UC-MSCs in a dose-dependent manner. <br><strong>Conclusions:</strong> The data suggested that metformin could enhance the expression of alkaline phosphatase and the osteogenic differentiation ability of MSCs derived from the umbilical cord.</p>}, number={-}, journal={Asian Medical Journal and Alternative Medicine}, author={Chadee, Nuengruethai and Suwanprateep, Jintamai and Tantrawatpan, Chairat and Kheolamai, Pakpoom and Tantikanlayaporn, Duangrat and Manochantr, Sirikul}, year={2022}, month={Aug.}, pages={S110} }