@article{Suwanmanee_Tantrawatpan_Kheolamai_Tantikanlayaporn_Manochantr_2022, title={Fucoxanthin Attenuates Hydrogen Peroxide-induced Oxidative Stress in Placenta-derived Mesenchymal Stem Cells through Antioxidant Activity}, volume={22}, url={https://asianmedjam.com/index.php/amjam/article/view/1330}, abstractNote={<p><strong>Introduction:</strong> Osteoporosis is a bone disease that results in a loss of bone mass. Oxidative stress promotes osteoblast apoptosis which leads to decrease bone formation. Fucoxanthin, a carotenoid extracted from brown seaweeds, has antioxidant activity through an increased antioxidant enzyme expression.</p> <p><strong>Objectives:</strong> This study attempts to explore the cytoprotective effect and antioxidant activity of fucoxanthin to promote the cell viability of placenta-derived mesenchymal stem cells (PL-MSCs) under oxidative stress conditions.</p> <p><strong>Methods:</strong> PL-MSCs were obtained from a pregnant woman after normal delivery. Following the characterization, PL-MSCs were treated with fucoxanthin (1-5 µM) and 750 µM H<sub>2</sub> O<sub>2</sub> for 24 h or cultured in both fucoxanthin (1-5 µM) and H<sub>2</sub> O<sub>2</sub> for 24 h. The cell viability, intracellular reactive oxygen species(ROS) production,superoxide dismutase (SOD) activity and glutathione (GSH) were determined following treatment.</p> <p><strong>Results:</strong> The resultsshowed that treatment and pretreatment with fucoxanthin increased cell viability, SOD activity, GSH levels of PL-MSCs when compare to control which cultured in 750 µM H<sub>2</sub> O<sub>2</sub> without fucoxanthin for 24 h. While, treatment and pretreatment with fucoxanthin decreased intracellular ROS production of PL-MSCs when compare to control which cultured in 750 µM H<sub>2</sub> O<sub>2</sub> without fucoxanthin for 24 hours.</p> <p><strong>Conclusions:</strong> This study demonstrated that the treatment and pretreatment with fucoxanthin increased the viability and increased antioxidant activity of PL-MSCs when compared with the control which cultured in 750 µM H<sub>2</sub> O<sub>2</sub> without fucoxanthin. In addition, the treatment with fucoxanthin decreased intracellular ROS production compared with the control. This study established that fucoxanthin can protect PL-MSCs against oxidative stress.</p>}, number={-}, journal={Asian Medical Journal and Alternative Medicine}, author={Suwanmanee, Gunticha and Tantrawatpan, Chairat and Kheolamai, Pakpoom and Tantikanlayaporn, Duangrat and Manochantr, Sirikul}, year={2022}, month={Aug.}, pages={S109} }