@article{Meesuk_Suwanprateeb_Kheolamai_Tantrawatpan_Tantikanlayaporn_Manochantr_2022, title={Inhibition of MicroRNAs Promotes Osteogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Human Placenta}, volume={22}, url={https://asianmedjam.com/index.php/amjam/article/view/1329}, abstractNote={<p><strong>Introduction:</strong> Mesenchymal stem cells (MSCs) are multipotent stem cells that have a regenerative function. They can be classified into several cell types, including osteoblasts, adipocytes, and chondrocytes. MSCs can be isolated from the placenta with a non-invasive procedure. However, the osteogenic differentiation potential of MSCs derived from the placenta (PL-MSCs) is not very efficient. The investigation for improving the osteogenic differentiation potential of PL-MSCs is important for the application of PL-MSCs in the clinical field. MicroRNA, including miR-21, miR-27b, miR-29a, and let-7b, plays an important role as a post-transcriptional regulator in various processes. However, there is restricted data regarding the role of miRNA on osteogenic differentiation of PL-MSCs.<br /><strong>Objectives:</strong> This study is intended to investigate the role of miR-21, miR-27b, miR-29a, and let-7b on the osteogenic differentiation of PL-MSCs. <br /><strong>Methods:</strong> MSCs were isolated from the placenta using enzymatic digestion. The expressions of miRNAs during osteogenic induction were measured using quantitative real-time PCR. After transfection with anti-miRNA, the osteogenic differentiation potential was determined using alkaline phosphatase activity, alizarin red staining, and osteogenic gene expression.<br /><strong>Results:</strong> The results showed that miR-21, miR-27b, miR-29a, and let-7b were down-regulated during osteogenic differentiation of PL-MSCs.After the transfection with anti-miRNAs, the ALP activity was significantly increased compared with PL-MSCs cultured in an osteogenic differentiation medium without anti-miRNAs, especially on days 21 and 28. In addition, alizarin red staining and the expression of osteogenic markers were up-regulated when compared with the control group.<br /><strong>Conclusions:</strong> The data obtained from this study demonstrated that the inhibition of miR-21, miR-27b, miR-29a, and let-7b could enhance the osteogenic differentiation potential of MSCs derived from the placenta.</p>}, number={-}, journal={Asian Medical Journal and Alternative Medicine}, author={Meesuk, Ladda and Suwanprateeb, Jintamai and Kheolamai, Pakpoom and Tantrawatpan, Chairat and Tantikanlayaporn, Duangrat and Manochantr, Sirikul}, year={2022}, month={Aug.}, pages={S139} }